Ultrasensitive mass-spectrometry analysis
Quantifying proteins in single cells at high-throughput with mass spectrometry
The sensitivity of mass spectrometry analysis depends on many factors, including sample preprations, data aquision, and data interpretaion. These factors can be benchmarked and optimized by methods developed in the Slavov Laboratory:
- Data-driven Optimization of Mass-Spectrometry (DO-MS)
- Data-driven Alignment of Retention Times for IDentification (DART-ID)
DO-MS and DART-ID are major contributors to the increased throughput and quantitative accuracy of SCoPE2 compared to Single-Cell ProtEomics by Mass Spectrometry (SCoPE-MS). Below are video presentaions of these methods by Gray Huffman and Albert Chen.