Prioritized single-cell proteomics
Prioritized single-cell proteomics (pSCoPE) was developed by Huffman et al, 2022. pSCoPE allows ensures duty-cycle time for analyzing prioritized peptides across all single cells (thus increasing data consistency) while analyzing identifiable peptides at full duty-cycle, thus increasing proteome depth. These strategies increased the quantified data points for challenging peptides and the overall proteome coverage about 2-fold.
pSCoPE is implemented as a module of MaxQuant.Live, starting with version 2.1 and is compatible with all Orbitrap instruments. All additional code needed for pSCoPE and reproducing Huffman et al., 2022 is available at github.com/SlavovLab/pSCoPE.